"FrameStar® plates led to significantly better results and reduced evaporation compared to standard PCR plates."

Dr. Andreas Dahl, MPI f. Molekulare Genetik, Berlin, Germany

Product Info

Product Information

#

Low profile, 0.1 ml polypropylene wells, polycarbonate frame, cut corner H12, working volume: <100 µl, total well capacity: 200 µl; designed for use on Roche LightCycler® 96 and 480 (with 96 well block); extra white wells for improved sensitivity in fluorescent assays

Our FrameStar® Roche Style plates are designed to achieve optimised assay conditions on the Roche LightCycler® 96 and 480 (with 96 well block).

This particular style of plate is in a low profile 96-well format; perfect for reaction volumes of 10-100 µl. The wells are shorter than "standard" profile wells, which decrease the "dead space" between the heated lid of the thermal cycler and the sample in the well. This eliminates condensation forming on the side wall of the tubes, preventing reduction in PCR volume and increasing the efficiency of the reaction. This is especially recommended for reaction volumes below 20 µl.

The extra white wells allow for increased sensitivity in fluorescent assays.

The semi-skirted frame allows for labelling or barcoding.

Technical Specifications

Plate Width127.70 ± 0.25 mm
Plate Depth85.48 ± 0.25 mm
Plate Height15.60 ± 0.25 mm
Well Depth15.10 ± 0.10 mm
Well Diameter5.50 ± 0.10 mm
Distance to centre of A1 from top edge11.24 ± 0.25 mm
Distance to centre of A1 from left edge14.38 ± 0.25 mm
Pitch (distance between A1 and A2)9.00 mm

Features

Plate Features

  • Compatible with the Roche LightCycler® 96 and 480 (with 96 well block)
  • Recommended for low volume PCR
  • Rigid polycarbonate frame adds to the mechanical stability of the plate - as a result thermal expansion and sample evaporation is reduced significantly, leading to an improved consistency in PCR results
  • Ultra-smooth, uniform, thin-walled polypropylene tubes for optimum PCR and real-time (RT-qPCR) results
  • Improved seal integrity due to thermal stability of frame
  • Compatible with standard multichannel pipettes
  • Ideal for use with robotic systems
  • <0.1 ml (100 µl) working volume, 0.2 ml (200 µl) total well capacity
  • Plates are available with a clear polycarbonate frame and extra white wells for improved sensitivity when using fluorescent based assays
  • Raised and colour differentiated grid reference to aid well and sample identification
  • Available barcoded


Application Data

Ct values of data run on a Roche LightCycler® 480, comparing 4titude FrameStar® Roche Style, High Sensitivity plates (4ti-0954) and Competitor R equivalent plates.
The new 4titude 4ti-0954 plate out performs the competitor plate with significantly earlier Ct values.

Barcoding

This plate is available with linear Code 128-format barcodes to aid identification and traceability for your samples. To order your plates with barcodes, simply add /SBC to the end of the ordering code for Standard Single Barcoding, /DBC for Standard Double Barcoding, /TBC for Standard Triple Barcoding, or /QBC for Standard Quadruple Barcoding (see Barcoding for more details).

Compatibility

Compatibility

MANUFACTURER

BLOCK TYPE

INSTRUMENT

ROCHE qPCR Cyclers (96 well block) LC96 | LC480


For recommended plate options please refer to the Instrument Compatibility Table.


Ordering

Ordering

FrameStar® 96 Well Semi-Skirted PCR Plate, Roche Style, High Sensitivity

Low profile, 0.1 ml polypropylene wells, polycarbonate frame, cut corner H12, working volume: <100 µl, total well capacity: 200 µl; designed for use on Roche LightCycler® 96 and 480 (with 96 well block); extra white wells for improved sensitivity in fluorescent assays

Code

Description

Plates/Case

4ti-0954 extra white wells, clear frame 50 plates

Combi Pack Code

Description

Plates & Seals/Case

4ti-0954/0560 4ti-0954 + qPCR Seal (4ti-0560) 50 plates + 50 seals

Sealing Options

Improved Sealing

There are a number of sealing options available to this plate, depending on the application and desired outcome.

Click here to find out more about our Adhesive Seal range >>

Click here to find out more about our Heat Seal range >>

Click here to find out more about our Cap Strip range >>

Click here to find out more about our Lids and Mats >>

See below for a selection:


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