"FrameStar® plates led to significantly better results and reduced evaporation compared to standard PCR plates."

Dr. Andreas Dahl, MPI f. Molekulare Genetik, Berlin, Germany

Product Info

Product Information

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30 µl polypropylene wells, skirted polycarbonate frame, cut corner A24; designed for use on standard thermal cyclers


Our FrameStar® 384 Well Skirted PCR Plates are designed for high-throughput PCR. They are compatible with the majority of 384 well block PCR, qPCR and sequencing instruments, and the rigid 2-component design eliminates warping and distortion during PCR making it ideal for use with robotic systems.

The skirt allows for labelling or barcoding.

Technical Specifications

Plate Width127.76 ± 0.25 mm
Plate Depth85.48 ± 0.25 mm
Plate Height10.60 ± 0.25 mm
Well Depth9.60 ± 0.10 mm
Well Diameter3.10 ± 0.10 mm
Distance to centre of A1 from top edge8.99 ± 0.25 mm
Distance to centre of A1 from left edge12.13 ± 0.25 mm
Pitch (distance between A1 and A2)4.50 mm

Features

Plate Features

  • Recommended for low volume PCR
  • Rigid polycarbonate frame adds to the mechanical stability of the plate - as a result thermal expansion and sample evaporation is reduced significantly, leading to an improved consistency in PCR results
  • Ultra-smooth, uniform, thin-walled polypropylene tubes for optimum PCR and real-time (RT, qPCR) results
  • Improved seal integrity due to thermal stability of the frame
  • Compatible with standard multichannel pipettes
  • Ideal for use with robotic systems
  • 30 µl working volume, 55 µl total well capacity
  • Compatible with the majority of 384 well block PCR, qPCR and sequencing instruments
  • Raised and colour differentiated grid reference to aid well and sample identification
  • Stackable
  • Available barcoded
  • 6 frame colour options for the clear well variety - purple, blue, clear, green, red, and black
  • Also available as a black frame with white wells (for optimum signal-to-noise ratio when using fluorescent based assays); as a black frame with black wells (for minimal light diffusion and interference); and as a clear frame with frosted wells (see below)

Now Available - Frosted Wells for use with ABI®/Life Technologies® qPCR Instruments

While clear wells are the best for sample visibility and optical appeal, white wells increase the signal-to-noise ratio by maximising reflect of light in fluorescent based assays used in real-time PCR and qPCR. This leads to a better sensitivity and reproducibility from a wide range of qPCR instruments from most manufacturers.

However, white well PCR plates may lead to software related problems with ABI®/Life Technologies® cyclers. For this reason the manufacturer recommends the use of plates with frosted wells as an alternative to white wells. Although the frosting does not provide the same sensitivity as white wells, readings are still considerably higher than those achieved with standard clear wells.

In response to this, we at 4titude® have developed a frosted well variety of the FrameStar® 384 well plate to offer ABI®/Life Technologies® instrument users the time and money-saving advantages of FrameStar®. And as the signal levels are directly comparable with ABI®/Life Technologies® plates, there's no need for users to recalibrate their instruments.

Find these plates in the Order tab under product code 4ti-0387.

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Frosted wells compared to clear wells

Barcoding

This plate is available with linear Code 128-format barcodes to aid identification and traceability for your samples. To order your plates with barcodes, simply add /SBC to the end of the ordering code for Standard Single Barcoding, /DBC for Standard Double Barcoding, /TBC for Standard Triple Barcoding, or /QBC for Standard Quadruple Barcoding (see Barcoding for more details).

Compatibility

Compatibility

MANUFACTURER

BLOCK TYPE

INSTRUMENT

ABI® / LIFE TECHNOLOGIES / THERMO FISHER SCIENTIFIC Thermal Cyclers (384 well block) GeneAmp® 9700 | Verity | Proflex | Multiblock system
qPCR Cyclers (384 well block) QuantStudio™ 5 / 6 / 7 / 12K | ViiA7™ | 7900 HT FAST
Sequencers (384 well block) 3100 | 3130XL | 3500 | 3500XL | 3730 | 3730XL
AGILENT / STRATAGENE Thermal Cyclers (384 well block) SureCycler 8800
ANALYTIK JENA / BIOMETRA Thermal Cyclers (384 well block) Flexcycler2 | T1 Thermocycler | Tadvanced | TProfessional | Trobot 96 |
qPCR Cyclers (384 well block) qTOWER3 84 / 84G
BIOER TECHNOLOGIES Thermal Cyclers (384 well block) Gene Touch 384
BIO-RAD Thermal Cyclers (384 well block) C1000 Touch | S1000
qPCR Cyclers (384 well block) CFX384 Touch
BIO-RAD MJ RESEARCH Thermal Cyclers (384 well block) PCT200™ / 220™ / 221™ / 225™ / 240™
CORBETT RESEARCH Thermal Cyclers (384 well block) (Qiagen) Palm Cycler 384
EPPENDORF Thermal Cyclers (384 well block) MasterCycler® ep 384 / Pro 384
GE HEALTHCARE / AMERSHAM Sequencers (384 well block) MegaBACE™ 4000
PEQLAB / VWR Thermal Cyclers (384 well block) peqSTAR 384X
SENSOQUEST Thermal Cyclers (384 well block) Labcycler
TECHNE Thermal Cyclers (384 well block) Prime | PrimeG | Prime Elite | Prime Elite Satellite | PCRmax Alpha cycler 1 / 2/ 4 | TC412 | TC512 | Genius | Genius Quad | Flexigene


For recommended plate options please refer to the Instrument Compatibility Table.


Ordering

Ordering

FrameStar® 384 Well Skirted PCR Plate

PP wells, PC frame, cut corner A24, working volume: 30 µl, total well capacity: 55 µl

Code

Description

Plates/Case

4ti-0384 clear wells, purple frame 50 plates
4ti-0384/B clear wells, blue frame
50 plates
4ti-0384/C clear wells, clear frame
50 plates
4ti-0384/G clear wells, green frame
50 plates
4ti-0384/R clear wells, red frame
50 plates
4ti-0384/X clear wells, black frame
50 plates
4ti-0385 white wells, black frame
50 plates
4ti-0386 black wells, black frame
50 plates
4ti-0387 frosted wells, clear frame 50 plates

Sealing Options

Sealing Options

There are a number of sealing options available to this plate, depending on the application and desired outcome.

Click here to find out more about our Adhesive Seal range >>

Click here to find out more about our Heat Seal range >>

Click here to find out more about our Cap Strip range >>

Click here to find out more about our Lids and Mats >>

    See below for a selection:


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