4LAB Automated PCR/qPCR Sample Preparation System
Downloads
Technical Specifications
Worktable Capacity:
2-3 x 96/384 PCR plates
1-2 x 96 tip racks (50/200µl)
Reagent area 1: 8 x 1.5/2ml microcentrifuge tubes
Reagent area 2: 6 x 2ml storage tubes (free standing) and 1 x 5ml bottle
Function:
Liquid (sample/reagent) transfer (LT)
Multiple Dispense (MD)
Serial Dilution (SD)
Hold (Pause)
Mixing (MIX)
Loop
Automated Pipetting Module:
Interchangeable single/8-channel, max. volume 50µl/200µl
Connection:
RS-232, USB2.0
Power Supply:
100-240V, 50/60Hz, 100W
Size (WxDxH):
590 x 440 x 460 mm
Weight (Nett):
25 Kg
Performance
50µl Tip on single or 8-channel pipetting module
| 1µl | 50µl | |
| Accuracy (Rel.) | ±7% | ±1% |
| Precision (Rel. CV) | ≤ 7.5% | ≤ 0.4% |
200µl Tip on single or 8-channel pipetting module
| 10µl | 200µl | |
| Accuracy (Rel.) | ±3% | ±0.8% |
| Precision (Rel. CV) | ≤ 1% | ≤ 0.15% |
Note: According to ISO-8655 standards (Gravimetric method), APM is calibrated in temperature (21-25°C, ±0.5°C) and humidity (60-90%) controlled environment. Twice-distilled water, robotic tips and microbalance were used.
Accessories
Used Tip Tray, Disposable, 4ti-2061
Elevated 384-well PCR Plate Adapter, 4ti-2053
20 x 1.5ml Tube Adapter, 4ti-2060
Elevated 96-well PCR Plate Adapter, 4ti-2052
Tip Rack Adapter, 4ti-2051
1.5ml or 2ml tube adapter (4x2), 4ti-2054
2ml (3x2) & 5ml (1) bottle adapter, 4ti-2055
8-channel pipetting reservoir, 4ti-2062
PCR/qPCR Sample Preparation
4titude's® 4LAB™ is an automated, high-precision pipetting system specifically designed for low-volume PCR/qPCR sample preparation. For acceptable PCR results, small volumes of reagents need to be dispensed accurately and consistently, which is tedious, time consuming and there are many chances for mistakes. It is available as a 4-position and a 6-position workstation.
The 4LAB™ was designed as a replacement for manual PCR/qPCR sample preparation. Accuracy, precision and consistency are guaranteed, and reagent wastage is reduced. Unlike complex, multi-purpose robotic systems, our instrument was designed for the researcher without prior robotic experience. Intuitive set-up and programming will save your laboratory time and money straight away.
Easy and Convenient to Use
- PC software can be mastered in an hour. No trained technician required.
- Built-in PCR/qPCR set-up protocols can be quqickly modified and transferred via USB memory stick.
- Single and 8-channel, 50µl or 200µl automated pipetting modules can be changed without tools.
- 4 interchangeable standard microplate / tip rack adapters and 2 interchangeable reagent vessel adapters available.
- Other applications include sequencing set-up, HLA typing and SNP detection, etc.
Affordable Solution
- The most affordable automated pipetting system available.
- Uses tips that are compatible with Beckman® Biomek® NX, FX & 3000.
- Cooling block adapters keep sensitive reagents / samples at 7°C for over an hour.
- Save reagent costs by human error reduction and using low volume PCR plates.
Easy to Service
- The automated pipetting modules are simple to remove and can be returned for service and calibration.
- PC software is upgraded via the internet.
- The entire system is compact and light-weighted.
Accurate and Precise
- Each automated pipetting module is calibrated to ISO-8655 standards.
- Excellent results for qPCR standard curve and replicates (Figure 1).
- Better Precision of pipetting results than manual pipetting (Figure 2).
Trademarks: Beckman®, Biomek® 3000, Biomek® FX, Biomek® NX (Beckman Coulter Inc.); Roche®, LightCycler® 480 (Roche Group); DyNAmo™ (Finnzyme Oy); SYBR® (Molecular Probes Inc.); Invitrogen™ Platinum® (Invitrogen Corp.)
| Code | Description | Details |
| 4ti-2000 | 4LAB™ 4-Position System | 1 |
| 4ti-2200 | 4LAB6 6-Position System | 1 |
| 4ti-2010 | 50µl Single-Channel Pipetting Module | 1 |
| 4ti-2020 | 50µl 8-Channel Pipetting Module | 1 |
| 4ti-2030 | 200µl Single-Channel Pipetting Module | 1 |
| 4ti-2040 | 200µl 8-Channel Pipetting Module | 1 |
| 4ti-2051 | 96-Tip Rack Adapter | 1 |
| 4ti-2052 | Elevated 96-well PCR Plate Adapter | 1 |
| 4ti-2053 | Elevated 384-well PCR Plate Adapter | 1 |
| 4ti-2054 | 1.5ml Tube Adapter (4x2) | 1 |
| 4ti-2055 | 2ml (3x2) and 5ml (1) Tube Adapter | 1 |
| 4ti-2056 | Cooling Block for 96-well PCR Plates | 1 |
| 4ti-2057 | Cooling Block for 384-well PCR Plates | 1 |
| 4ti-2058 | Cooling Block for 1.5ml Tubes (4x2) | 1 |
| 4ti-2059 | Cooling Block for 2ml (3x2) and 5ml (1) Tubes | 1 |
| 4ti-2060 | 20x1.5ml Tube Adapter | 1 |
| 4ti-2061 | Used Tip Tray, Disposable | 10 Trays |
| 4ti-2062 | 8-Channel Pipetting Reservoir | 1 |
| 4ti-2063 | Deep-Well Plate Adapter | 1 |
| 4ti-2064 | Reservoir (80ml) | 20 Reservoirs |
| 4ti-2100 | 50μl Pipette Tip, Non-Sterile | 10x96 |
| 4ti-2101 | 200μl Pipette Tip, Non-Sterile | 10x96 |
| 4ti-2102 | 50μl Pipette Tip, Sterile | 10x96 |
| 4ti-2013 | 200μl Pipette Tip, Sterile | 10x96 |
