Heat Sealing Parameters for 4titude heat sealing films and foils. 


Catalogue Number Name Dwell time and temperature for
a 96 well plate
Dwell time and temperature for
a 348 Well Plate
4ti-0521 Peel-Seal 175°C – 3sec 175°C – 2sec
4ti-0531 Pierce-Seal 175°C – 2.5sec 175°C – 2sec
4ti-0533 Pierce-Seal Strong 175°C – 2.5sec 175°C – 2sec
4ti-0536 Foil-Seal 175°C – 2.5sec 175°C – 2sec
4ti-0541 Clear-Seal 170°C – 2sec 170°C – 2sec
4ti-0571 &
4ti-0573
Clear Weld Seal & Mk. II version 170°C – 2sec 170°C – 2sec
4ti-0580 Clear-Seal 3730 170°C – 2sec 170°C – 1.5sec
4ti-0591 Thermal Bond 175°C – 2.5sec 175°C – 2sec

 

These values are for guidance only. It is advisable to conduct individual trials depending on the plate type being sealed.

These values are for sealing with the 4titude 4Sealer  (Cat. No. 4ti-0600). If using an automated sealer, reduce the dwell time by 0.5 - 1.0 second.

Certain seals such as the Peel-Seal are prone to curling prior to sealing. To overcome this issue, place a weighted metal sheet (4ti-0601) over the seal prior to sealing to keep the seal flat. If this item is being used, increase the dwell time by 1 second.

 

 

 
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