Sealing Parameters

Technical data and parameters for heat sealing laboratory plates:

Product Code	Product Name	Temperature & dwell time for 96 well PCR plates*	Temperature & dwell time for 384 Well PCR Plates*	Temperature & dwell time for Vision Plates™
4ti-0541	Clear Seal	175-185°C, 2-3 seconds	165-180°C, 3 seconds	185-200°C, 3 seconds
4ti-0575	Clear Weld Seal Mark II	175-180°C, 2-3 seconds	170-175°C, 2-3 seconds	-
4ti-0581	Clear Seal 3730	165-175°C, 3 seconds	165-175°C, 2 seconds	175-185°C, 2-3 seconds
4ti-0521	Peel Seal	175-185°C, 3 seconds	170-175°C, 2-3 seconds	-
4ti-0587	DMSO Resistant Peel Seal	175-185°C, 3 seconds	170-175°C, 2-3 seconds	-
4ti-0531	Pierce Seal	160-175°C, 2 seconds	160-175°C, 2 seconds	185-200°C, 3 seconds
4ti-0536	Foil Seal	165-180°C, 2 seconds	160-175°C, 2-3 seconds	185-200°C, 3 seconds
4ti-0547	PS Foil Seal	-	-	185-200°C, 3 seconds
4ti-0527	Black Seal	-	-	185-200°C, 3 seconds
4ti-0591	Thermal Bond	170-180°C, 2-3 seconds	160-170°C, 2 seconds	-
4ti-0595	Gas Permeable Heat Seal	165-175°C, 3 seconds	165-175°C, 3 seconds	175-185°C, 3 seconds

*These values are for two-piece PCR plates.  For one-piece polypropylene PCR plates, increase dwell time by 1 second.

These values are for guidance only. It is advisable to conduct individual trials depending on the plate type being sealed. These values are for sealing with the semi automatic 4titude® 4s2™ Thermal Sealer (4ti-0650). If using an automated sealer, reduce the dwell time by 0.5 - 1.0 second.

Certain seals such as the Peel Seal are prone to curling prior to sealing. To overcome this issue, place a weighted metal platen (4ti-0602) over the seal prior to sealing to keep the seal flat. If this item is being used, increase the dwell time by 1 second.