Is manual sealing and peeling of your microplates limiting your throughput?

Manual seal application and removal can lead to problems in your workflow such as unwanted evaporation, cross contamination and damage to consumables, not to mention using up precious man-hours.

4titude® and Brooks offer purpose made instrumentation to help you automate and streamline these processes. For a limited time, we are offering special promotions on these well-established instruments to help get your lab set up for automation!

Product Info

Product Information

  • Unrivalled sealing performance and consistency -
    5,000 perfect seals without intervention
  • SiLA compatible - Easy integration into robotic systems
  • No external air supply required - Cost-saving and compact device, simple installation

The main advantage of the a4S is that it does not require an air supply, which gives it the flexibility to be used on standard lab benches as a stand-alone unit, or integrated within a robotic set‑up. The sealer’s rapid heating element enables a fast start-up, and the stand-by function saves energy when the unit is not in use.

The a4S sealer is compatible with a wide range of SBS footprint plates, including all PCR plate formats from 96 well to 1536 well, assay plates, deep well storage plates and microplates. Varying plate heights, from 3 mm to 60 mm can be accommodated with minimal adjustments of the instrument. Plates are presented in portrait format and the plate position on the shuttle allows good access for robotic integration.

Technical Specifications

Dimensions (W x L x H)230 x 507 x 276 mm
Sealing Temperature Range100-195 °C
Sealing Time Range0.1-10 sec
Weight (without roll)27 kg
Power SupplyV in: AC 100-240 V
V out: DC 24 V 320 W
Power Consumption700 W (max)
Working Temperature Range10-30 °C
Operating Humidity (RH)0-85%
ConnectionRS-232 serial port, USB port

Rolls with a width of between 75-85 mm can be loaded in either of two roll positions, depending on roll length and available space, enabling the entire instrument to fit under the gantree of most liquid handling robots.

The width and length of the applied seals are set to precisely cover an SBS footprint plate without interfering with plate stacking. The seal position can be adjusted to give an overhang at one end of the plate to facilitate ease of seal removal.


Instrument Features

Simple Installation

  • No need for compressed air - Installation is fast and flexible
  • 2 positions for different roll sizes - Increased flexibility
  • Optional roll cover - Seal protection for sensitive applications

Easy Handling

  • Colour touch screen with intuitive user interface - Ease of use
  • Unlimited password protected protocols - 3 security levels save personalised and SOP set temperatures and times
  • Rapid heating - Fast start up time, block uniformity is maintained to ± 1 °C
  • Variable time/temperature controls - Adjustment of time and temperature settings enables the perfect seal to be established for any sealer friendly, high-quality plate type, e.g. all 4titude® plates
  • Up to 5,000 seals without manual intervention - True walk-away-system

Reproducible Sealing

  • Reproducible sealing - Fixed pressure and accurate time and temperature controls ensure consistent sealing
  • High sealing pressure - Improved seal uniformity on the widest range of plates
  • Sealing cycle time of less than 15 sec - Time saving

Highest Flexibility

  • Compatible with a wide range of plate types, from racked tubes to 1536 well PCR plates - Highest flexibility

Economic Efficiency

  • Competitive pricing - Save money without compromises
  • Auto standby mode - Saves energy and prolongs component life
  • 2 year warranty on parts and labour
  • Extended warranty and service contracts available - For peace of mind

Plug and Play Robotic Integration

All 4titude® instruments are compliant with SiLA standards for rapid integration of automated systems ( This means they can be "plug and play" connected with other instruments, such as readers, robotic arms and liquid handlers, to give a custom automated system without the need to write expensive custom drivers. The a4S heat sealer is extremely versatile leaving you the freedom to expand and configure your systems such as adding robotic plate handling.

• Full communication protocol available - Operate the instrument and record sealing conditions for each seal using the RS232 communication port

• SiLA compatible - Quick and easy integration with other SiLA compliant devices using the SiLA driver (available at extra cost, please see ordering information).


Heat Sealing Applications

Heat sealing is the gold standard method for plate sealing. Not only does it minimise sample loss but also maximises sample security for PCR, qPCR, storage, and other applications.

Three typical applications for automated heat sealing are outlined below. Please click here to see our entire portfolio of heat seals.

qPCR - Reduce evaporation and save money

The optimised sealing performance of the a4S heat sealer prevents reagent volume loss by evaporation.

Up to a fivefold reduction in the costs of sealing can be achieved when heat sealing with the a4S compared to using caps or adhesive seals - Reliable qPCR results and significant reagent savings.

  • Clear Weld Heat Seal Mark 2 is particularly designed for Hydrocyclers

Cell assays and cell culture - Increase cell viability and assay performance

Heat sealing is the optimal method for cell based assay plates and cell culture plates. It is a proven method for compound storage, particularly in protective gases such as Argon and CO₂.

Sample Storage - Strongest resistance against organic solvents

Essential requirements for long-term storage include maintaining sample integrity and minimising the risks of sample degradation.

  • DMSO Resistant Peel Heat Seal - The first 100% DMSO resistant seal. Pure DMSO can be stored at room temperature for 12 months with this seal with no deterioration of the seal quality.

Challenge Us - Tailor-Made Solutions

4titude® stocks one of the widest ranges of sealing solutions for varying applications. We can also design and manufacture custom solutions for specific non-standard sealing requirements. This service includes custom sealer blocks for the a4S and 4s3™ heat sealers as well as custom size and specification seal material. Contact us at



4titude® a4S Automatic Roll Heat Sealer Introduction

Watch this short video to see how easy to use our a4S Automatic Roll Heat Sealer really is



Service Documents



a4S Automatic Roll Heat Sealer

For automated heat sealing of microplates, PCR plates, assay plates and storage plates used in medium to high-throughput or full automation applications




4ti-0665 Includes: universal power supply, power cord, plate support adapters A (4ti-0665-2) and B (4ti-0665-3), 24 month parts and labour warranty


4ti-0665-1* a4S Seal Loading Tool
4ti-0665-2* a4S Plate Support Adapter A
4ti-0665-3* a4S Plate Support Adapter B
4ti-0665-4* a4S Roll Holder Set; includes spindle, clamping wheels, locking nuts, O-rings
4ti-0665-5* a4S Vacuum Cups, Front; clear, set of 2
4ti-0665-6* a4S Vacuum Cups, Back; black, set of 2 2
4ti-0665-7 Plastic Tweezers 1
4ti-0665-8 a4S Clear Plastic Roll Dust Cover; Please Note: only applicable for smaller rolls (product code 4ti-xxxx/80, /100, /122)
4ti-0665-41 SiLA driver for a4S heat sealer
1 license
4ti-0665-11 Warranty extension, third year (36 months total) 1
4ti-0665-12 Warranty extension, third and fourth year (48 months total) 1
4ti-0665-113 Warranty extension, fourth year 1

*These items are included with the a4S heat sealer (Code 4ti-0665)

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