"FrameStar® plates led to significantly better results and reduced evaporation compared to standard PCR plates."

Dr. Andreas Dahl, MPI f. Molekulare Genetik, Berlin, Germany

Product Info

Product Information

Technical Drawing of FrameStar® 96 Well Skirted Optical Bottom PCR Plate


Low profile, flat optical bottom, 0.1 ml clear polypropylene wells, clear polycarbonate frame, cut corner H1, working volume: <100 µl, total well capacity: 180 µl

We have now extended our FrameStar® range to include PCR plates with optically clear flat bottom wells for microscopy applications.

The FrameStar® 96 Well Skirted Optical Bottom PCR Plates (next to their counterpart within our Random Access range, 4ti-0970/RA) are currently unique in the market, being suitable for use in both microscopy and PCR. Optical bottom plates are ideal for applications requiring single cell sorting followed by molecular biology techniques such as (q)PCR and sequencing.

The flat bottoms enable excellent stackability, making these plates well suited for small sample volume storage such as compound libraries, with no risk of damaging the seal of the plate below. Additionally, the small well volume enables excellent sample recovery.

Technical Specifications

Plate Width127.76 ± 0.25 mm
Plate Depth85.48 ± 0.25 mm
Plate Height16.10 ± 0.25 mm
Well Depth12 ± 0.10 mm
Well Diameter5.46 ± 0.10 mm
Distance to centre of A1 from top edge11.24 ± 0.25 mm
Distance to centre of A1 from left edge14.38 ± 0.25 mm
Pitch (distance between A1 and A2)9.00 mm

The low profile wells of this plate are shorter than "standard" profile wells, which decreases the "dead space" between the heated lid of the thermal cycler and the reagents in the well. This reduces condensation forming on the side wall of the tubes, moderating changes in PCR volume and increasing the efficiency of the reaction. This is especially recommended for reaction volumes below 20 µl.

The rigid polycarbonate skirt of this design eliminates warping and distortion during PCR, and makes it ideal for use with robotic systems.

Figure 1 illustrates the differences in tube format between the standard conical PCR tube and the flat bottom tubes. The surface area of the optical base is 7.74 mm2, with a thickness of 0.5 mm. The total volume of a conical well is 0.2 ml compared to 0.18 ml for flat bottom tubes.

The primary target application for optical bottom plates is assays that require the use of microscopy, for example, work with single cells including fluorescent imaging and cell sorting.

Figure 1. Technical drawing of a conical PCR tube (A) compared to a flat bottom PCR tube (B)

Features

Plate Features

  • Suitable for microscopy and small volume sample storage
  • Recommended for low volume PCR
  • Rigid polycarbonate frame adds to the mechanical stability of the plate - as a result thermal expansion and sample evaporation is reduced significantly, leading to an improved consistency in PCR results
  • Ultra-smooth, uniform, thin-walled polypropylene tubes for optimum PCR and real-time (RT-qPCR) results
  • Improved seal integrity due to thermal stability of frame
  • Compatible with standard multichannel pipettes
  • Ideal for use with robotic systems
  • <0.1 ml (100 µl) working volume, 0.18 ml (180 µl) total well capacity
  • Colour differentiated grid reference to aid well and sample identification
  • Stackable
  • Available barcoded

Barcoding

This plate is available with linear Code 128-format barcodes to aid identification and traceability of your samples. To order your plates with barcodes, simply add /SBC to the end of the ordering code for Standard Single Barcoding, /DBC for Standard Double Barcoding, /TBC for Standard Triple Barcoding, or /QBC for Standard Quadruple Barcoding (see Barcoding for more details).

Compatibility

Compatibility

MANUFACTURER

BLOCK TYPE

INSTRUMENT

AGILENT / STRATAGENE Thermal Cyclers (96 well block) Robocycler Gradient
qPCR Cyclers (96 well block) AriaMx
ANALYTIK JENA / BIOMETRA Thermal Cyclers (96 well block) Flexcycler2 | T1 Thermocycler | T1 Thermocycler | Tgradient | Tone | Tadvanced | TProfessional (Standard / Basic) Gradient / XL | Trobot 96 | SpeedCycler2 (SP, SPR)
BIO-RAD Thermal Cyclers (96 well block) C1000 Touch | S1000
qPCR Cyclers (96 well block) CFX96 Touch | CFX 96 Touch Deep Well | CFX connect
BIO-RAD MJ RESEARCH Thermal Cyclers (96 well block) PCT100™ / 200™ / 220™ / 221™ / 225™ / 240™
qPCR Cyclers (96 well block) Chromo4™ | Opticon2™
EPPENDORF Thermal Cyclers (96 well block) MasterCycler® ep / ep gradient / Pro / Pro S / nexus / nexus gradient / nexus SX1 / nexus GSX1
qPCR Cyclers (96 well block) MasterCycler™ ep realplex
GE HEALTHCARE / AMERSHAM Sequencers (96 well block) MegaBACE™ 500 | MegaBACE™ 1000 mark 2
PEQLAB / VWR Thermal Cyclers (96 well block) peqSTAR 96X
SENSOQUEST Thermal Cyclers (96 well block) Labcycler
TECHNE Thermal Cyclers (96 well block) TC412 | TC512 | Genius | Genius Quad | Touchgene | Touchgene Gradient | Flexigene
qPCR Cyclers (96 well block) Quantica


For recommended plate options please see the Instrument Compatibility Table and reference the FrameStar® 96 Well Skirted PCR Plate (4ti-0960).


Ordering

Ordering

FrameStar® 96 Well Skirted Optical Bottom PCR Plate

Low profile, flat optical bottom, 0.1 ml clear polypropylene wells, clear polycarbonate frame, cut corner H1, working volume: <100 µl, total well capacity: 180 µl

Code

Description

Plates/Case

4ti-0970 clear wells, clear frame
50 plates

Sealing Options

Sealing Options

There are a number of sealing options available to this plate, depending on the application and desired outcome.

Click here to find out more about our Heat Seal range >>

Click here to find out more about our Adhesive Seal range >>

Click here to find out more about our Cap Strip range >>

Click here to find out more about our Lids and Mats >>

See below for a selection:


Related Products