Product Info

Product Information

Technical Drawing of Random Access 96 Well Skirted Flat Bottom PCR Plate, 2D Coded

96 individual tubes in plate format, low profile, flat bottom 2D coded, 0.1 ml clear polypropylene tubes, white rigid polycarbonate frame, linear barcoded, cut corner H1, working volume: <100 µl, total well capacity: 180 µl

96 well, fully skirted, low profile format with each tube inserted separately into the plate frame, allowing for selection and removal of individual tubes from the plate. Each tube clicks into place in the frame ensuring tubes are secure once inserted.

We have now extended our Random Access range to include PCR plates with 2D coded flat bottoms for applications that require superior sample tracking.

In applications where it is essential for samples to be tracked throughout their processing, e.g. for diagnostic assays on clinical samples, tube labelling is a far safer and reliable method compared to cap or seal labelling (which can be misplaced, damaged or rendered unreadable after piercing). Our plates have a unique 2D code printed on the bottom of the well and a linear barcode on the side of the plate to allow for identification of both the plate and individual tubes within.

Technical Specifications

Plate Width 127.76 ± 0.25 mm
Plate Depth 85.48 ± 0.25 mm
Plate Height 15.50 ± 0.25 mm
Well Depth 12.2 ± 0.10 mm
Well Diameter 5.46 ± 0.10 mm
Distance to centre of A1 from top edge 11.24 ± 0.25 mm
Distance to centre of A1 from left edge 14.38 ± 0.25 mm
Pitch (distance between A1 and A2) 9.00 mm
Size of Code 12 x 12 px
2 x 2 mm
Format white on black
Content 8-digit numeric

The rigid polycarbonate skirt of this design eliminates warping and distortion during PCR. Together with the SBS footprint, this makes the plate suitable for use with robotic systems, ideal for both small to high throughput labs.

Empty frames are available for tubes to be transferred to. The frames can be re-used multiple times, depending on the application.

Sample tubes can be identified by the unique 2D code on the base of the wells, removed from the Random Access plate quickly and easily, and moved to another Random Access frame for downstream processing or storage. Quick selection of desired tubes reduces the time needed for the sample plate to be out of the freezer and therefore reduces the chance of other wells defrosting in the meantime.

Flat bottom PCR plates can be stacked, allowing for optimal use of freezer space as multiple plates can be housed in the same space as a storage rack. The 2D codes on the bottom of the wells can be read by most 2D datamatrix readers including FluidX Whole Rack Scanners. The codes are highly scratch resistant and can withstand cold storage (-80 °C), temperatures up to 100 °C and solvents such as DMSO. 2D datamatrix codes utilise data redundancy so even if codes are partly destroyed, the information will still be retained. Each code is tested for readability and guaranteed to be unique.

Figure 1 illustrates the differences in tube format between the standard conical PCR tube and the flat bottom tubes. The surface area of the optical base is 7.74 mm2, with a thickness of 0.5 mm. The total volume of a conical well is 0.2 ml compared to 0.18 ml for flat bottom tubes.

Figure 1. Technical drawing of a conical RA PCR tube (A) compared to a flat bottom RA PCR tube (B)

Features

Plate Features

  • 96-well tubes are individually removable from the rigid frame
    - Removal of single tubes (samples) from the plate while the rest can remain in storage, reducing freeze-thawing of the whole plate
  • Thin walled tubes made from PP
    - Optimal results in (q)PCR whilst also being suitable for storage and incubation
    - Fewer transfer steps as the same tube can be used for both (q)PCR and storage
  • Each tube can be individually heat sealed using the Random Access Pierce Heat Seal Strong (see below)
    - The contents of each tube is left secure from cross contamination and evaporation for storage and transfer
    - Each tube can still be separated after sealing
  • Alternatively, each tube can be individually sealed using the Cap Mat for PCR Plates
    - As a temporary solution when samples need to be repeatedly accessed
    - Each tube can still be separated after sealing
  • Suitable for small volume sample storage, allowing for optimal use of freezer space as multiple plates can be housed in the same space as a storage rack
  • These plates are labelled with linear Code 128-format barcodes to aid identification and traceability for your samples.
  • Rigid polycarbonate frame provides mechanical stability of the plate
    - Ideal for use with robotic systems
  • <100 µl working volume, <180 µl total well capacity
  • Alphanumeric grid reference to aid well and sample identification
  • Empty frames are available for tubes to be transferred to. Frames can be re-used multiple times, depending on the application


Sealing Options

Manual Random Access Heat Sealing

The 96 well Random Access plate can be sealed in one step using Random Access Pierce Seal Strong (4ti-05381/RA), 96 individual foil seal spots on a removable backing. These seals result in individually sealed tubes that are pierceable, allowing for sample access. Random Access Heat Seals are currently available in sheet format for use with the 4s3™ Semi-Automatic Sheet Heat Sealer (using the 4ti-0613 Random Access adapter).

Random Access Sealing Procedure using the 4s3™ Semi-Automatic Sheet Heat Sealer
(4ti-0655)


Coming Soon - Automatic Random Access Heat Sealing

The roll-fed a4SRA Automatic Random Access Heat Sealer allows for automatic sealing of Random Access plates using Random Access Heat Seal rolls. The roll with the indexed groups of 96 individual sealing discs is automatically fed through the heat sealer. The accurate sealing is controlled by a sensor which gets activated by optical windows in the material feed, but can also by adjusted. Sealing temperature, time of sealing and exit delay (for cooling) can be controlled via the instrument's touchscreen. Custom versions of the instrument and sealing material are possible.

Random Access Sealing Procedure using the a4SRA Automatic Random Access Heat Sealer
(4ti-0675, prototype shown)


Customisation

4titude® also offers customisation of the Random Access Plates such as colour coding of the individual wells. Please contact us for details.

Compatibility

Compatibility

MANUFACTURER

BLOCK TYPE

INSTRUMENT

AGILENT / STRATAGENE Thermal Cyclers (96 well block) Robocycler Gradient
qPCR Cyclers (96 well block) AriaMx
BIO-RAD Thermal Cyclers (96 well block) C1000 Touch | S1000
qPCR Cyclers (96 well block) CFX96 Touch | CFX96 Touch Deep Well | CFX connect
BIO-RAD MJ RESEARCH Thermal Cyclers (96 well block) PCT100™ / 200™ / 220™ / 221™ / 225™ / 240™
qPCR Cyclers (96 well block) Chromo4™ | Opticon2™
EPPENDORF Thermal Cyclers (96 well block) MasterCycler® ep / ep gradient / Pro / Pro S / nexus / nexus gradient / nexus SX1 / nexus GSX1
qPCR Cyclers (96 well block) MasterCycler™ ep realplex
PEQLAB / VWR Thermal Cyclers (96 well block) peqSTAR 96X
SENSOQUEST Thermal Cyclers (96 well block) Labcycler
TECHNE Thermal Cyclers (96 well block) TC412 | TC512 | Genius | Genius Quad | Touchgene | Touchgene Gradient | Flexigene
qPCR Cyclers (96 well block) Quantica


For recommended plate options please see the Instrument Compatibility Table and reference the Random Access 96 Well Skirted PCR Plate (4ti-0960/RA).


Videos

Videos

4titude Random Access Plates & Seals Introduction

Watch this short video and find out how to seal Random Access Plates with individual pierce seals with our Heat Sealer 4s3™ and learn more about our exciting new range of Random Access products.

Ordering

Ordering

Random Access 96 Well Skirted Flat Bottom PCR Plate, 2D Coded

96 individual tubes in plate format, low profile, flat bottom 2D coded, 0.1 ml clear polypropylene tubes, white rigid polycarbonate frame, linear barcoded, cut corner H1, working volume: <100 µl, total well capacity: 180 µl

Code

Description

Plates/Case

4ti-0975/RA clear tubes, white frame 50 plates

Random Access 96 Well Skirted Frame, White

Rigid PC frame, cut corner H1, linear barcoded

Code

Description

Frames/Case

4ti-0960/RA-F*white frame10 frames

*Please note, /SBC must be added to 4ti-0960/RA-F to include a standard single barcode.

Sealing Options

Sealing Options

Random Access seals are available to match the 96 individual tubes of the Random Access 96 Well Skirted Flat Bottom PCR Plate, 2D Coded.


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