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FrameStar® 384 Well Skirted PCR Plate
Round 96 Deep Well Storage Plates for use with Magnetic Separators
1.5 and 2.0 ml Microcentrifuge Tubes
Low Binding Products
96 individual tubes in plate format, low profile, flat bottom 2D coded, 0.1 ml clear polypropylene tubes, white rigid polycarbonate frame, linear barcoded, cut corner H1, working volume: <100 µl, total well capacity: 180 µl
Available in the following colours
96 well, fully skirted, low profile format with each tube inserted separately into the plate frame, allowing for selection and removal of individual tubes from the plate. Each tube clicks into place in the frame ensuring tubes are secure once inserted.
We have now extended our Random Access range to include PCR plates with 2D coded flat bottoms for applications that require superior sample tracking.
In applications where it is essential for samples to be tracked throughout their processing, e.g. for diagnostic assays on clinical samples, tube labelling is a far safer and reliable method compared to cap or seal labelling (which can be misplaced, damaged or rendered unreadable after piercing). Our plates have a unique 2D code printed on the bottom of the well and a linear barcode on the side of the plate to allow for identification of both the plate and individual tubes within.
The rigid polycarbonate skirt of this design eliminates warping and distortion during PCR. Together with the SBS footprint, this makes the plate suitable for use with robotic systems, ideal for both small to high throughput labs.
Empty frames are available for tubes to be transferred to. The frames can be re-used multiple times, depending on the application.
Sample tubes can be identified by the unique 2D code on the base of the wells, removed from the Random Access plate quickly and easily, and moved to another Random Access frame for downstream processing or storage. Quick selection of desired tubes reduces the time needed for the sample plate to be out of the freezer and therefore reduces the chance of other wells defrosting in the meantime.
Flat bottom PCR plates can be stacked, allowing for optimal use of freezer space as multiple plates can be housed in the same space as a storage rack. The 2D codes on the bottom of the wells can be read by most 2D datamatrix readers including FluidX Whole Rack Scanners. The codes are highly scratch resistant and can withstand cold storage (-80 °C), temperatures up to 100 °C and solvents such as DMSO. 2D datamatrix codes utilise data redundancy so even if codes are partly destroyed, the information will still be retained. Each code is tested for readability and guaranteed to be unique.
Figure 1 illustrates the differences in tube format between the standard conical PCR tube and the flat bottom tubes. The surface area of the optical base is 7.74 mm2, with a thickness of 0.5 mm. The total volume of a conical well is 0.2 ml compared to 0.18 ml for flat bottom tubes.
Figure 1. Technical drawing of a conical RA PCR tube (A) compared to a flat bottom RA PCR tube (B)
The 96 well Random Access plate can be sealed in one step using Random Access Pierce Seal Strong (4ti-05381/RA), 96 individual foil seal spots on a removable backing. These seals result in individually sealed tubes that are pierceable, allowing for sample access.
Random Access Heat Seals are currently available in sheet format for use with the
4s3™ Semi-Automatic Sheet Heat Sealer (using the 4ti-0613 Random Access adapter).
Random Access Sealing Procedure
using the 4s3™ Semi-Automatic Sheet Heat
The roll-fed a4SRA Automatic Random Access Heat Sealer allows for automatic sealing of Random Access plates using Random Access Heat Seal rolls.
The roll with the indexed groups of 96 individual sealing discs is automatically fed through the heat sealer. The accurate sealing is controlled by a sensor which gets activated by optical windows in the material feed, but can also by adjusted. Sealing temperature, time of sealing and exit delay (for cooling) can be controlled via the instrument's touchscreen.
Custom versions of the instrument and sealing material are possible.
Random Access Sealing Procedure
using the a4SRA Automatic Random Access Heat
(4ti-0675, prototype shown)
4titude® also offers customisation of the Random Access Plates such as colour coding of the individual wells. Please contact us for details.
For recommended plate options please see the Instrument Compatibility Table and reference the Random Access 96 Well Skirted PCR Plate (4ti-0960/RA).
Watch this short video and find out how to seal Random Access Plates with individual pierce seals with our Heat Sealer 4s3™ and learn more about our exciting new range of Random Access products.
*Please note, /SBC must be added to 4ti-0960/RA-F to include a standard single barcode.
Find out more