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FrameStar® 384 Well Skirted PCR Plate
Round 96 Deep Well Storage Plates for use with Magnetic Separators
1.5 and 2.0 ml Microcentrifuge Tubes
Low Binding Products
96 individual tubes in plate format, low profile, flat optical bottom, 0.1 ml clear polypropylene tubes, black rigid polycarbonate frame, cut corner H1, working volume: <100 µl, total well capacity: 180 µl
Available in the following colours
96 well, fully skirted, low profile format with each tube inserted separately into the plate frame, allowing for selection and removal of individual tubes from the plate. Each tube clicks into place in the frame ensuring tubes are secure once inserted.
We have now extended our Random Access range to include PCR plates with optically clear flat bottom wells for microscopy applications.
The Random Access 96 Well Skirted Optical Bottom PCR Plates (next to their counterpart within our FrameStar® range, 4ti-0970) are currently unique in the market, being suitable for use in both microscopy and PCR, for example, when single cell sorting is followed by molecular biology applications such as qPCR and sequencing.
Due to their flat bottoms and stackability, these plates are well suited for small sample volume storage (such as compound libraries), with no risk of damaging the seal of of the plate below. Additionally, the small well volume enables excellent sample recovery.
The rigid polycarbonate skirt of this design eliminates warping and distortion during PCR. Together with the SBS footprint, this makes the plate suitable for use with robotic systems, ideal for both small to high throughput labs.
Empty frames are available for tubes to be transferred to. The frames can be re-used multiple times, depending on the application.
Figure 1 illustrates the differences in tube format between the standard conical PCR tube and the flat bottom tubes. The surface area of the optical base is 7.74 mm2, with a thickness of 0.5 mm. The total volume of a conical well is 0.2 ml compared to 0.18 ml for flat bottom tubes.
The primary target application for optical bottom plates is assays that require the use of microscopy, for example, work with single cells including fluorescent imaging and cell sorting.
Figure 1. Technical drawing of a conical RA PCR tube (A) compared to a flat bottom RA PCR tube (B)
The 96 well Random Access plate can be sealed in one step using Random Access Pierce Seal Strong (4ti-05381/RA), 96 individual foil seal spots on a removable backing. These seals result in individually sealed tubes that are pierceable, allowing for sample access.
Random Access Heat Seals are currently available in sheet format for use with the
4s3™ Semi-Automatic Sheet Heat Sealer (using the 4ti-0613 Random Access adapter).
Random Access Sealing Procedure
using the 4s3™ Semi-Automatic Sheet Heat
The roll-fed a4SRA Automatic Random Access Heat Sealer allows for automatic sealing of Random Access plates using Random Access Heat Seal rolls.
The roll with the indexed groups of 96 individual sealing discs is automatically fed through the heat sealer. The accurate sealing is controlled by a sensor which gets activated by optical windows in the material feed, but can also by adjusted. Sealing temperature, time of sealing and exit delay (for cooling) can be controlled via the instrument's touchscreen.
Custom versions of the instrument and sealing material are possible.
Random Access Sealing Procedure
using the a4SRA Automatic Random Access Heat
(4ti-0675, prototype shown)
This plate is available with linear Code 128-format barcodes to aid identification and traceability of your samples. To order your plates with barcodes, simply add /SBC to the end of the ordering code for Standard Single Barcoding, /DBC for Standard Double Barcoding, /TBC for Standard Triple Barcoding, or /QBC for Standard Quadruple Barcoding (see Barcoding for more details).
4titude® also offers customisation of the Random Access Plates such as colour coding of the individual wells. Please contact us for details.
For recommended plate options please see the Instrument Compatibility Table and reference the Random Access 96 Well Skirted PCR Plate (4ti-0960/RA).
Watch this short video and find out how to seal Random Access Plates with individual pierce seals with our Heat Sealer 4s3™ and learn more about our exciting new range of Random Access products.
Find out more