Product Info

Product Information

Technical Drawing of Random Access 96 Well Skirted Optical Bottom PCR Plate

96 individual tubes in plate format, low profile, flat optical bottom, 0.1 ml clear polypropylene tubes, black rigid polycarbonate frame, cut corner H1, working volume: <100 µl, total well capacity: 180 µl

96 well, fully skirted, low profile format with each tube inserted separately into the plate frame, allowing for selection and removal of individual tubes from the plate. Each tube clicks into place in the frame ensuring tubes are secure once inserted.

We have now extended our Random Access range to include PCR plates with optically clear flat bottom wells for microscopy applications.

The Random Access 96 Well Skirted Optical Bottom PCR Plates (next to their counterpart within our FrameStar® range, 4ti-0970) are currently unique in the market, being suitable for use in both microscopy and PCR, for example, when single cell sorting is followed by molecular biology applications such as qPCR and sequencing.

Due to their flat bottoms and stackability, these plates are well suited for small sample volume storage (such as compound libraries), with no risk of damaging the seal of of the plate below. Additionally, the small well volume enables excellent sample recovery.

Technical Specifications

Plate Width 127.76 ± 0.25 mm
Plate Depth 85.48 ± 0.25 mm
Plate Height 15.50 ± 0.25 mm
Well Depth 12 ± 0.10 mm
Well Diameter 5.46 ± 0.10 mm
Distance to centre of A1 from top edge 11.24 ± 0.25 mm
Distance to centre of A1 from left edge 14.38 ± 0.25 mm
Pitch (distance between A1 and A2) 9.00 mm

The rigid polycarbonate skirt of this design eliminates warping and distortion during PCR. Together with the SBS footprint, this makes the plate suitable for use with robotic systems, ideal for both small to high throughput labs.

Empty frames are available for tubes to be transferred to. The frames can be re-used multiple times, depending on the application.

Figure 1 illustrates the differences in tube format between the standard conical PCR tube and the flat bottom tubes. The surface area of the optical base is 7.74 mm2, with a thickness of 0.5 mm. The total volume of a conical well is 0.2 ml compared to 0.18 ml for flat bottom tubes.

The primary target application for optical bottom plates is assays that require the use of microscopy, for example, work with single cells including fluorescent imaging and cell sorting.

Figure 1. Technical drawing of a conical RA PCR tube (A) compared to a flat bottom RA PCR tube (B)

Features

Plate Features

  • 96-well tubes are individually removable from the rigid frame
    - Removal of single tubes (samples) from the plate while the rest can remain in storage, reducing freeze-thawing of the whole plate
  • Thin walled tubes are made from PP
    - Optimal results in (q)PCR whilst also being suitable for storage and incubation
    - Fewer transfer steps as the same tube can be used for microscopy, (q)PCR and storage
  • Suitable for microscopy and small volume sample storage
  • Each tube can be individually heat sealed using the Random Access Pierce Heat Seal Strong (see below)
    - The contents of each tube is left secure from cross contamination and evaporation for storage and transfer
    - Each tube can still be separated after sealing
  • Rigid polycarbonate frame provides mechanical stability of the plate
    - Ideal for use with robotic systems
  • <100 µl working volume, <180 µl total well capacity
  • Alphanumeric grid reference to aid well and sample identification
  • Empty frames are available for tubes to be transferred to. Frames can be re-used multiple times, depending on the application


Sealing Options

Manual Random Access Heat Sealing

The 96 well Random Access plate can be sealed in one step using Random Access Pierce Seal Strong (4ti-05381/RA), 96 individual foil seal spots on a removable backing. These seals result in individually sealed tubes that are pierceable, allowing for sample access. Random Access Heat Seals are currently available in sheet format for use with the 4s3™ Semi-Automatic Sheet Heat Sealer (using the 4ti-0613 Random Access adapter).

Random Access Sealing Procedure using the 4s3™ Semi-Automatic Sheet Heat Sealer
(4ti-0655)


Coming Soon - Automatic Random Access Heat Sealing

The roll-fed a4SRA Automatic Random Access Heat Sealer allows for automatic sealing of Random Access plates using Random Access Heat Seal rolls. The roll with the indexed groups of 96 individual sealing discs is automatically fed through the heat sealer. The accurate sealing is controlled by a sensor which gets activated by optical windows in the material feed, but can also by adjusted. Sealing temperature, time of sealing and exit delay (for cooling) can be controlled via the instrument's touchscreen. Custom versions of the instrument and sealing material are possible.

Random Access Sealing Procedure using the a4SRA Automatic Random Access Heat Sealer
(4ti-0675, prototype shown)


Barcoding

This plate is available with linear Code 128-format barcodes to aid identification and traceability of your samples. To order your plates with barcodes, simply add /SBC to the end of the ordering code for Standard Single Barcoding, /DBC for Standard Double Barcoding, /TBC for Standard Triple Barcoding, or /QBC for Standard Quadruple Barcoding (see Barcoding for more details).


Customisation

4titude® also offers customisation of the Random Access Plates such as colour coding of the individual wells. Please contact us for details.

Compatibility

Compatibility

MANUFACTURER

BLOCK TYPE

INSTRUMENT

AGILENT / STRATAGENE Thermal Cyclers (96 well block) Robocycler Gradient
qPCR Cyclers (96 well block) AriaMx
BIO-RAD Thermal Cyclers (96 well block) C1000 Touch | S1000
qPCR Cyclers (96 well block) CFX96 Touch | CFX96 Touch Deep Well | CFX connect
BIO-RAD MJ RESEARCH Thermal Cyclers (96 well block) PCT100™ / 200™ / 220™ / 221™ / 225™ / 240™
qPCR Cyclers (96 well block) Chromo4™ | Opticon2™
EPPENDORF Thermal Cyclers (96 well block) MasterCycler® ep / ep gradient / Pro / Pro S / nexus / nexus gradient / nexus SX1 / nexus GSX1
qPCR Cyclers (96 well block) MasterCycler™ ep realplex
PEQLAB / VWR Thermal Cyclers (96 well block) peqSTAR 96X
SENSOQUEST Thermal Cyclers (96 well block) Labcycler
TECHNE Thermal Cyclers (96 well block) TC412 | TC512 | Genius | Genius Quad | Touchgene | Touchgene Gradient | Flexigene
qPCR Cyclers (96 well block) Quantica


For recommended plate options please see the Instrument Compatibility Table and reference the Random Access 96 Well Skirted PCR Plate (4ti-0960/RA).


Videos

Videos

4titude Random Access Plates & Seals Introduction

Watch this short video and find out how to seal Random Access Plates with individual pierce seals with our Heat Sealer 4s3™ and learn more about our exciting new range of Random Access products.

Ordering

Ordering

Random Access 96 Well Skirted Optical Bottom PCR Plate

96 individual tubes in plate format, low profile, flat optical bottom, 0.1 ml clear polypropylene tubes, black rigid polycarbonate frame, cut corner H1, working volume: <100 µl, total well capacity: 180 µl

Code

Description

Plates/Case

4ti-0970/RA clear tubes, black frame 50 plates

Random Access 96 Well Skirted Frame, Black

Rigid PC frame, cut corner H1

Code

Description

Frames/Case

4ti-0970/RA-Fblack frame10 frames

Sealing Options

Sealing Options

Random Access seals are available to match the 96 individual tubes of the Random Access 96 Well Skirted Optical Bottom PCR Plate.


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