White Well qPCR Plates & Strips

We all know qPCR is an excellent technique to quantify your DNA but what if there was a way to make your reactions even more sensitive? White PCR consumables can offer a helping hand in those difficult situations where you’re just not getting the sensitivity you need from your experiments.


Well colour options - A question of sensitivity

There are many aspects to your qPCR consumables that can affect the quality of your results, and the colour of your wells is a big one!

Most qPCR instruments detect fluorescence through the top of the well and when using clear tubes, loss of signal through the tube wall reduces the amount of signal returning to the detector.

White wells are opaque, significantly reducing signal loss through tube walls and maximising reflection. This feature is particularly important for detecting targets with low expression levels as signals are likely to be low.

Fig 1. Greater sensitivity in a qPCR reaction enables earlier Ct values and higher fluorescence readings

White well plates give higher sensitivity

Comparison of identical reactions between plates with either clear, frosted or white wells highlights that white wells outperform clear and frosted wells with both earlier Ct values and higher overall end fluorescence values.

Earlier Ct values are particularly beneficial for reducing false positives, especially where the Ct value of the gene of interest is greater than 38. It is well understood that earlier Ct values are more reliable and accurate than later Ct values due to the potential presence of primer-dimers.

Fig 2. Identical qPCR in plates with clear, frosted and white wells (4ti-0770/C, 4ti-0771, and 4ti-0772, respectively). Clear and frosted wells perform similarly, whereas white wells gave earlier Ct values and higher fluorescence intensity

White well plates give higher consistency

When using clear PCR tubes, contamination and dirt on the well surface of thermal cycler heat blocks can have a major factor affect on fluorescent readings by altering the uniformity of reflection between wells. White wells are not affected by this issue due to their opaque properties resulting to far greater consistency between wells.


4titude® offer white well plates in a range of different formats to suit most qPCR instruments on the market. They are available in many different formats to meet your needs for instrument compatibility, throughput and automation. For extra flexibility, we also have a range of tube strips available with white wells.


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FrameStar® 384 Well Plates (2-Component Plates)

4ti-0385 FrameStar® 384 Well Skirted PCR Plate
n/a A24 white wells, black frame 50 plates Webpage
4ti-0381 FrameStar® 384 Well Skirted PCR Plate, Roche Style
n/a A24, P24 white wells, clear frame 50 plates Webpage


FrameStar® 96 Well Plates (2-Component Plates)

4ti-0961 FrameStar® 96 Well Skirted PCR Plate
Low H1 white wells, black frame 50 plates Webpage
4ti-0901 FrameStar® 96 Well Semi-Skirted PCR Plate
Standard H1 white wells, clear frame 50 plates Webpage
4ti-0951 FrameStar® 96 Well Semi-Skirted PCR Plate, Roche Style
Low H12 white wells, clear frame 50 plates Webpage
4ti-0954 FrameStar® 96 Well Semi-Skirted PCR Plate, Roche Style, High Sensitivity Low H12 extra white wells, clear frame 50 plates Webpage
4ti-0771 FrameStar® 96 Well Semi-Skirted PCR Plate, ABI® Style
Standard A12 white wells, black frame 50 plates Webpage
4ti-0911 FrameStar® 96 Well Semi-Skirted PCR Plate, ABI® FastPlate Style
Low A1 white wells, clear frame 50 plates Webpage
4ti-0731 FrameStar® 96 Well Semi-Skirted PCR Plate With Upstand, ABI® Style
Standard A12 white wells, black frame 50 plates Webpage
4ti-0711 FrameStar® 96 Well Non-Skirted PCR Plate
Standard H1 white wells, black frame 50 plates Webpage
4ti-0721 FrameStar® 96 Well Non-Skirted PCR Plate, Low Profile
Low H1 white wells, black frame 50 plates Webpage


96 Well Plates (1-Component Plates)

4ti-0741 96 Well Skirted PCR Plate
Low H1 white plate 50 plates Webpage
4ti-0761 96 Well Semi-Skirted PCR Plate
Standard A12 white plate 50 plates Webpage
4ti-0955 96 Well Semi-Skirted PCR Plate, Roche Style
Low H12 white plate 50 plates Webpage
4ti-0750/W 96 Well Non-Skirted PCR Plate
Standard H12 white plate 50 plates Webpage


Breakable Plates (2-Component & 1-Component Dividable Plates)

4ti-1001 FrameStar® Break-A-Way PCR Plate
Standard A12 white wells, black frame
50 plates Webpage
4ti-1201 FrameStar® Break-A-Way PCR Plate, Low Profile
Low A12 white wells, black frame 50 plates Webpage
4ti-0750/W/TA Tear-A-Way™ 96/8 PCR Plate
Standard H12 white plate 50 plates Webpage


Vari-Strips™ and Vari-Plates™ (Detachable Strips)

4ti-0753 Vari-Strip™ 8 Well PCR Tube Strips, Low Profile Low n/a white wells 120 strips Webpage
4ti-0753/950W Vari-Plate™ 96 Well Semi-Skirted Frame, Roche Style Low H12 white wells, white frame 50 plates Webpage
4ti-0753/757 Vari-Plate™ 96 Well Skirted Frame Low H1 white wells, white frame 50 plates Webpage


FrameStrips® (2-Component Strips)

4ti-0785/XW FrameStrip® 8 Well PCR Tube Strips, Plus Domed Cap Strips Standard n/a white wells, black frame 120 strips Webpage
4ti-0786/XW FrameStrip® 8 Well PCR Tube Strips, Plus Flat Optical Cap Strips Standard n/a white wells, black frame 120 strips Webpage