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FrameStar® 384 Well Skirted PCR Plate
Round 96 Deep Well Storage Plates for use with Magnetic Separators
1.5 and 2.0 ml Microcentrifuge Tubes
Low Binding Products
We all know qPCR is an excellent technique to quantify your DNA but what if there was a way to make your reactions even more sensitive? White PCR consumables can offer a helping hand in those difficult situations where you’re just not getting the sensitivity you need from your experiments.
There are many aspects to your qPCR consumables that can affect the quality of your results, and the colour of your wells is a big one!
Most qPCR instruments detect fluorescence through the top of the well and when using clear tubes, loss of signal through the tube wall reduces the amount of signal returning to the detector.
White wells are opaque, significantly reducing signal loss through tube walls and maximising reflection. This feature is particularly important for detecting targets with low expression levels as signals are likely to be low.
Fig 1. Greater sensitivity in a qPCR reaction enables earlier Ct values and higher fluorescence readings
Comparison of identical reactions between plates with either clear, frosted or white wells highlights that white wells outperform clear and frosted wells with both earlier Ct values and higher overall end fluorescence values.
Earlier Ct values are particularly beneficial for reducing false positives, especially where the Ct value of the gene of interest is greater than 38. It is well understood that earlier Ct values are more reliable and accurate than later Ct values due to the potential presence of primer-dimers.
Fig 2. Identical qPCR in plates with clear, frosted and white wells (4ti-0770/C, 4ti-0771, and 4ti-0772, respectively). Clear and frosted wells perform similarly, whereas white wells gave earlier Ct values and higher fluorescence intensity
When using clear PCR tubes, contamination and dirt on the well surface of thermal cycler heat blocks can have a major factor affect on fluorescent readings by altering the uniformity of reflection between wells. White wells are not affected by this issue due to their opaque properties resulting to far greater consistency between wells.
4titude® offer white well plates in a range of different formats to suit most qPCR instruments on the market. They are available in many different formats to meet your needs for instrument compatibility, throughput and automation. For extra flexibility, we also have a range of tube strips available with white wells.